《植物生理学报》 2021, 57(2): 373-384

牡丹PsGRP基因克隆及转基因拟南芥的耐涝性分析

刘慧春，张加强，周江华，许雯婷，朱开元^*

浙江省园林植物与花卉研究所, 杭州311251

通信作者：朱开元；E-mail: 52011648@qq.com

摘　要：

富含甘氨酸蛋白(glycine-rich proteins, GRPs)是一类由甘氨酸高度重复序列组成的蛋白质, 在植物生长发育及应对逆境胁迫的过程中发挥着重要的作用。利用RACE技术从牡丹叶片中克隆获得了PsGRP基因的全长cDNA序列, 通过生物信息学分析发现, 该序列全长为844 bp, 含有一个长度为489 bp的开放阅读框, 编码162个氨基酸。序列比对结果显示, 牡丹PsGRP基因编码蛋白与胡桃(Juglans regia)、红小豆(Vigna angularis)和川桑(Morus notabilis)的GRP蛋白的相似度分别达到了83%、 74%和52%。将该基因转化拟南芥后进行耐涝性试验, 发现超表达PsGRP基因的拟南芥植株抗涝能力明显优于野生型植株。该研究结果将为进一步开展牡丹耐涝性的分子育种提供参考依据。

关键词： 牡丹; PsGRP基因; 转基因拟南芥; 耐涝

收稿：2020-10-10   修定：2021-01-05

资助：浙江省公益技术应用研究计划项目(LGN18C150007和 LGN19C150010)、浙江省农业科学院公共发展专项(617- CF0101G)和杭州市科技计划引导项目(20191231Y158)

Cloning of PsGRP gene from Paeonia suffruticosa and waterlogging tolerance analysis of transgenic Arabidopsis

LIU Huichun, ZHANG Jiaqiang, ZHOU Jianghua, XU Wenting, ZHU Kaiyuan^*

Zhejiang Institute of Landscape Plants and Flowers, Hangzhou 311251, China

Corresponding author: ZHU Kaiyuan; E-mail: 52011648@qq.com

Abstract:

Glycine-rich proteins (GRPs) are a class of proteins composed of highly repetitive glycine sequences, which play important roles in plant growth, development and stress resistance. The full-length
cDNA sequence of PsGRP gene was cloned from Paeonia suffruticosa leaves by RACE technology. Bioinformatics analysis showed that the full length of the sequence was 844 bp, containing an open reading frame of 489 bp, encoding 162 amino acids. Sequence alignment showed that the similarity of peony PsGRP with GRP proteins from Juglans regia, Vigna angularis and Morus notabilis was 83%, 74% and 52%, respectively. Then the PsGRP gene was transformed into Arabidopsis thaliana, and the waterlogging tolerance test was performed. The results indicated that PsGRP transgenic Arabidopsis plants enhanced waterlogging tolerance than wild-type plants. These results will provide strategies for peony molecular breeding about waterlogging tolerance.

Key words: Paeonia suffruticosa; PsGRP gene; transgenic Arabidopsis; waterlogging tolerance